Engineers develop new metabolic pathway to more efficiently convert sugars into biofuels

Sep 30, 2013 by Matthew Chin
Photo of colonies of E. coli that have been genetically modified by UCLA engineers using a new synthetic metabolic pathway. Credit: UCLA

(Phys.org) —UCLA chemical engineering researchers have created a new synthetic metabolic pathway for breaking down glucose that could lead to a 50 percent increase in the production of biofuels.

The new pathway is intended to replace the natural metabolic pathway known as glycolysis, a series of chemical reactions that nearly all organisms use to convert sugars into the molecular precursors that cells need. Glycolysis converts four of the six carbon atoms found in glucose into two-carbon molecules known acetyl-CoA, a precursor to biofuels like ethanol and butanol, as well as fatty acids, amino acids and pharmaceuticals. However, the two remaining glucose carbons are lost as carbon dioxide.

Glycolysis is currently used in biorefinies to convert sugars derived from plant biomass into biofuels, but the loss of two carbon atoms for every six that are input is seen as a major gap in the efficiency of the process. The UCLA research team's synthetic glycolytic pathway converts all six glucose carbon atoms into three molecules of acetyl-CoA without losing any as carbon dioxide.

The research is published online Sept. 29 in the peer-reviewed journal Nature.

The principal investigator on the research is James Liao, UCLA's Ralph M. Parsons Foundation Professor of Chemical Engineering and chair of the chemical and biomolecular engineering department. Igor Bogorad, a graduate student in Liao's laboratory, is the lead author.

"This pathway solved one of the most significant limitations in biofuel production and biorefining: losing one-third of carbon from carbohydrate raw materials," Liao said. "This limitation was previously thought to be insurmountable because of the way glycolysis evolved."

This synthetic pathway uses enzymes found in several distinct pathways in nature.

The team first tested and confirmed that the new pathway worked in vitro. Then, they genetically engineered E. coli bacteria to use the synthetic pathway and demonstrated complete carbon conservation. The resulting acetyl-CoA molecules can be used to produce a desired chemical with higher carbon efficiency. The researchers dubbed their new hybrid pathway non-oxidative , or NOG.

"This is a fundamentally new cycle," Bogorad said. "We rerouted the most central and found a way to increase the production of acetyl-CoA. Instead of losing carbon atoms to CO2, you can now conserve them and improve your yields and produce even more product."

The researchers also noted that this new synthetic pathway could be used with many kinds of sugars, which in each case have different numbers of per molecule, and no carbon would be wasted.

"For biorefining, a 50 percent improvement in yield would be a huge increase," Bogorad said. "NOG can be a nice platform with different sugars for a 100 percent conversion to acetyl-CoA. We envision that NOG will have wide-reaching applications and will open up many new possibilities because of the way we can conserve carbon."

The researchers also suggest this new pathway could be used in biofuel production using photosynthetic microbes.

The paper's other author is Tzu-Shyang Lin, who recently received a bachelor's degree from UCLA in chemical engineering.

Explore further: Novel technology produces gasoline by metabolically-engineered microorganism

More information: Synthetic non-oxidative glycolysis enables complete carbon conservation, DOI: 10.1038/nature12575

Related Stories

Microbe processes carbon via new metabolic pathway

Jan 21, 2011

(PhysOrg.com) -- A Dead Sea microbe has been found to use a previously unknown metabolic pathway to metabolize fats as a source of carbon to synthesize carbohydrates. This suggests there may be other undiscovered pathways ...

Recommended for you

New method to analyse how cancer cells die

18 hours ago

(Phys.org) —A team from The University of Manchester – part of the Manchester Cancer Research Centre - have found a new method to more efficiently manufacture a chemical used to monitor cancer cells.

The anti-inflammatory factory

Apr 22, 2014

Russian scientists, in collaboration with their colleagues from Pittsburgh University, have discovered how lipid mediators are produced. The relevant paper was published in Nature Chemistry. Lipid mediators are molecules that p ...

User comments : 4

Adjust slider to filter visible comments by rank

Display comments: newest first

Birger
3 / 5 (2) Sep 30, 2013
"a precursor to biofuels like ethanol and butanol"
I would recommend butanol as the final product. Ethanol has water dissolved in it, making fuel tanks rust.
Kiwini
1 / 5 (10) Sep 30, 2013
"a precursor to biofuels like ethanol and butanol"
I would recommend butanol as the final product. Ethanol has water dissolved in it, making fuel tanks rust.


Not quite... although the EPA-mandated gasahol is moisture-free when it leaves the blending facility, the ethanol portion will absorb water from the air, and when it gets to a high enough concentration will separate out, and then drop to the bottom of whatever vessel it is in. If it's in a storage tank that's designed for fuel but not water, in time you'll see pinholes developing wherever the water is sitting.

Even if your fuel tank is corrosion proof, many other parts that live downstream are not.

http://www.mossmo...nol.html
PPihkala
5 / 5 (1) Sep 30, 2013
I think it is a major step to eliminate the CO2 production part from this sugar conversion pathway.
Humpty
1 / 5 (8) Oct 01, 2013
Keep your tank FULL, to STOP the daily air intake and expulsion cycle as the air inside the tank contracts and expands with the variation of environmental temperature, and use it up quickly.

Simple.

But the full conversion of glucose into fuel - that is a significant step and it's probably good.

Fuck knows what will happen when the germs escape though.

More news stories

Computer program could help solve arson cases

Sifting through the chemical clues left behind by arson is delicate, time-consuming work, but University of Alberta researchers teaming with RCMP scientists in Canada, have found a way to speed the process.

Genetic code of the deadly tsetse fly unraveled

Mining the genome of the disease-transmitting tsetse fly, researchers have revealed the genetic adaptions that allow it to have such unique biology and transmit disease to both humans and animals.

Ocean microbes display remarkable genetic diversity

The smallest, most abundant marine microbe, Prochlorococcus, is a photosynthetic bacteria species essential to the marine ecosystem. An estimated billion billion billion of the single-cell creatures live i ...