Flexible throughout life by varying numbers of chromosome copies

Aug 14, 2013
The yeast Saccharomyces cerevisiae, which normally occurs as a single cell, has the ability to form colonies as it is able to duplicate single chromosomes.

Baker's yeast is a popular test organism in biology. Yeasts are able to duplicate single chromosomes reversibly and thereby adapt flexibly to environmental conditions. Scientists from the Luxembourg Centre for Systems Biomedicine (LCSB) of the University of Luxembourg, in collaboration with colleagues from the US Institute for Systems Biology (ISB) in Seattle, have now systematically studied the genetics of this process, which biologists refer to as aneuploidy. The team's new insights will allow a new medical evaluation of aneuploidy, which is associated with certain diseases when it occurs in multicellular organisms. Their results appear in the scientific journal PNAS.

Chromosomes are built out of the genetic material DNA and proteins. They are duplicated before a cell divides and then shared between the . In , the cell division cycle takes only 1.5 hours – meaning yeast have a very rapid succession of generations. That makes them capable of adapting very quickly to changing environmental conditions. The yeast Saccharomyces cerevisiae, which normally occurs as a single cell, has the ability to form colonies featuring multicellular structures with divided responsibilities, meaning the cells differentiate to perform different tasks. "The genetic basis for this change in the yeast's external appearance has remained unknown until now," says Dr. Alexander Skupin, a major contributor to the cooperative project.

Detailed have now shown that the yeast cells individually multiply as many as six of their 16 total chromosomes during cell division, and can reverse this multiplication again. "The organization of cell colonies and phenotypic switching between different types of colonies becomes a lot more flexible and rapid with reversible aneuploidy than if it depended on in the genes," Skupin says. "The duplication of a single chromosome is enough to change the yeast from a relatively smooth colony to one with what we describe as a 'fluffy' morphology."

If the cells reduce the copy number of this specific chromosome again – say upon another change in environmental conditions – then they turn back into a smooth colony. "So, the flexibility of yeast cells does not arise from the activity or inactivity of a single gene," project head at ISB, Dr. Aimée Dudley, explains. Rather gene dosage, which depends on the number of chromosome copies, is responsible for this: "If the chromosome is multiplied, then more copies of the same gene will be transcribed. That means it has a stronger effect than if it is active only once on one chromosome in the cell," Dudley concludes. These cells can change very rapidly.

The researchers now intend to find out what molecular mechanisms result from the activity of a gene transcribed from multiplied chromosomes. "Only then can we understand in detail how the different cell colonies arise," Skupin asserts.

Prof. Dr. Rudi Balling, director of LCSB, explains the medical implications of the new insights: "We have long known of aneuploidy in . One well-known example is Down's syndrome, in which the 21st chromosome, or part thereof, exists in triplicate. Recently, however, the phenomenon has also been observed in cancer cells and has even been connected with brain development. Given the rapid succession of generations in yeast, we can use it as a model organism – and study the mechanisms of in much greater detail to find out whether we can derive from it new approaches for diagnosing and treating human diseases."

Explore further: Researchers discover new strategy germs use to invade cells

More information: Tana, Z. et al. Aneuploidy underlies a multicellular phenotypic switch, PNAS 2013. www.pnas.org/cgi/doi/10.1073/pnas.1301047110

Related Stories

Family trees for yeast cells

May 13, 2013

Researchers at the Institute for Systems Biology in Seattle and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of ...

New key mechanism in cell division discovered

May 18, 2012

Researchers from the Bellvitge Biomedical Research Institute (IDIBELL) have identified the mechanism by which protein Zds1 regulates a key function in mitosis, the process that occurs immediately before cell division. The ...

Recommended for you

Researchers discover new strategy germs use to invade cells

Aug 20, 2014

The hospital germ Pseudomonas aeruginosa wraps itself into the membrane of human cells: A team led by Dr. Thorsten Eierhoff and Junior Professor Dr. Winfried Römer from the Institute of Biology II, members of the Cluster ...

Progress in the fight against harmful fungi

Aug 20, 2014

A group of researchers at the Max F. Perutz Laboratories has created one of the three world's largest gene libraries for the Candida glabrata yeast, which is harmful to humans. Molecular analysis of the Candida ...

How steroid hormones enable plants to grow

Aug 19, 2014

Plants can adapt extremely quickly to changes in their environment. Hormones, chemical messengers that are activated in direct response to light and temperature stimuli help them achieve this. Plant steroid ...

User comments : 0