New analysis shows how proteins shift into working mode

Aug 09, 2013 by Mike Ross
This 3-D figure of the enzyme dihydrofolate reductase (dhfr) shows the nine different areas where a small fluctuation in one part of this flexible molecule causes a sequence of atomic movements to propagate like falling dominoes. A new computer algorithm, CONTACT, identified these areas, which are colored red, yellow, green, orange, salmon, grey, light blue, dark blue and purple.

In an advance that will help scientists design and engineer proteins, a team including researchers from SLAC and Stanford has found a way to identify how protein molecules flex into specific atomic arrangements required to catalyze chemical reactions essential for life.

The achievement, published Sunday (Aug. 4, 2013) in Nature Methods, uses a new computer algorithm to analyze data from X-ray studies of crystallized proteins. Scientists were able to identify cascades of atomic adjustments that shift into new shapes, or conformations.

"Proteins need to move around to do their part in keeping the organism alive," said Henry van den Bedem, first author on the paper and a researcher with the Structure Determination Core of the Joint Center for Structural Genomics (JCSG) at the SSRL Directorate of SLAC. "But often these movements are very subtle and difficult to discern. Our research is aimed at identifying those fluctuations from X-ray data and linking them to a 's biological functions. Our work provides important new insights, which will eventually allow us to re-engineer these molecular machines."

Protein primer

Proteins are large molecules composed of a large number of up to 20 different amino acid units linked into chains, branches and loops. In , each protein typically adopts many different conformations, with each one exposing a specific section of the molecule and making it available for chemical activity. Changing the protein's atomic structure through , for instance, can interfere with critical chemical reactions, leading to disease or even death. On the other hand, drugs are often designed to promote or block certain protein fluctuations, so understanding the exact mechanism behind the fluctuations is very important.

This video is not supported by your browser at this time.
SLAC's Henry van den Bedem describes how his new computer algorithm identifies the ways that flexible protein molecules move so they can catalyze the chemical reactions essential for life. Credit: SLAC Multimedia Communications

For more than 50 years, scientists around the world have combined experimental techniques such as X-ray crystallography and with computer algorithms to determine the three-dimensional structure of proteins.

Each experimental technique has its strengths and limitations.

X-ray crystallography pinpoints the precise locations of atoms within the molecule, typically at extremely low temperatures, and it yields a static picture of the naturally floppy protein molecule. It doesn't show the many important conformations the molecule adopts when immersed in the warm, liquid environment of a living organism.

Nuclear magnetic resonance reveals the bonding patterns of a protein operating in a liquid environment at room temperature, but averages all of its conformations rather than providing details about each one.

The new technique combines the best of both worlds. Based on X-ray data collected at room temperature, scientists identified regions where cascades of conformational changes take place, and confirmed that those same regions can be found independently in NMR data.

This series of six figures illustrates the process by which the new CONTACT computer algorithm determines the pathway of atomic adjustments that occur due to small fluctuations in a flexible protein molecule, the enzyme cyclophilin A. In the first frame, the red-tinted molecular segment was moved slightly, causing a conflict – indicated by a green-dotted line – with nearby atoms (colored gold). To alleviate a conflict, CONTACT computationally selects alternate atomic positions observed in the X-ray data (second frame). But that move creates its own, new clash (third frame). A second move (fourth frame) erases the second conflict, but creates a third (fifth frame) which is relieved by yet another move (sixth frame). CONTACT repeats this "clash-and-relieve" procedure – like a chain of falling dominoes – until it reaches a stable conformation without conflicts. Credit: James Fraser/University of California, San Francisco

New computer algorithm

Central to the new technique is a new , called CONTACT, that analyzes protein structures determined by room temperature X-ray crystallography. Built upon an earlier algorithm created by van den Bedem, CONTACT detects how subtle features in the experimental data produced by changing conformations propagate through the protein and identifies regions within the protein where these cascades of small changes are likely to result in stable conformations.

The researchers tested CONTACT on two enzymes – proteins that catalyze . One, cyclophilin A, can facilitate protein folding, while the other, dihydrofolate reductase (dhfr), helps regulate cell proliferation and cell growth. Their method, for the first time, provides an atomically detailed structural framework for the dhfr NMR measurements, thus bridging the traditional divide between static, single X-ray and dynamic, averaged NMR structures.

Explore further: SANS: a unique technique to look inside plants' leaves

More information:… full/nmeth.2592.html

add to favorites email to friend print save as pdf

Related Stories

A new tool to reveal structure of proteins

Mar 19, 2012

A new method to reveal the structure of proteins could help researchers understand biological molecules – both those involved in causing disease and those performing critical functions in healthy cells.

Salk scientists add new bond to protein engineering toolbox

Aug 06, 2013

Proteins are the workhorses of cells, adopting conformations that allow them to set off chemical reactions, send signals and transport materials. But when a scientist is designing a new drug, trying to visualize the processes ...

NMR advance brings proteins into the open

Jun 24, 2013

When working a cold case, smart investigators try something new. By taking a novel approach to nuclear magnetic resonance spectroscopy – a blending of four techniques – scientists have been able to resolve ...

Recommended for you

SANS: a unique technique to look inside plants' leaves

7 hours ago

Plants' leaves capture the sunlight and convert it into the energy used to produce nutrients for their activities. This process is accomplished thanks to the presence of the thylakoid membrane system. While ...

Silver shines as antibacterial for medical implants

Mar 24, 2015

There have been growing concerns in the global health care system about the eradication of pathogens in hospitals and other patient-care environments. Overuse of antibiotics and antimicrobial agents has contributed ...

Fat turns from diabetes foe to potential treatment

Mar 24, 2015

A new weapon in the war against type 2 diabetes is coming in an unexpected form: fat. Researchers have discovered a new class of potentially therapeutic lipids, called fatty-acid esters of hydroxy fatty acids (FAHFAs). These ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.