New method for mapping the protein signals between healthy and diseased cells

Jul 02, 2013
Microscope image of human breast cancer cells (red) grown together with mouse fibroblasts (green). This mixed-species culture was used to validate the ability of the CTAP methodology to distinguish proteins from each cell population. Credit: Nicholas Gauthier, Chris Sander, Martin Miller

Researchers at Memorial Sloan-Kettering Cancer Center in New York, working in collaboration with researchers at the Proteome Center Tuebingen (PCT), have developed a new method for identifying the cell of origin of intracellular and secreted proteins within multicellular environments. This technological advancement is particularly exciting because it will provide investigators with a new tool for comprehensive mapping of cell-cell communication, which is especially important in all aspects of cancer development, maintenance, and response to therapy. For example, this method could be used to study cell signaling events between normal and malignant cells in order to better understand the molecular mechanisms by which surrounding normal cells alter tumor growth and response to treatment.

The technique, named cell type specific labeling using amino acid precursors (CTAP), exploits the inability of vertebrate cells to synthesize normally required for growth and homeostasis. A team headed by Dr. Nicholas Gauthier and Dr. Martin Miller at the Memorial Sloan-Kettering Cancer Center engineered cells to express amino acid biosynthesis enzymes, which enabled cells to grow on their own supply of amino acids produced from supplemented precursors.

The team went on to show that supplementing heavy stable isotope-labeled forms of these precursors led to incorporation of heavy amino acids into proteins produced in enzyme expressing cells. Dr. Boumediene Soufi and Dr. Boris Macek from the PCT designed experiments that utilized quantitative to search for proteins that contained these stable isotope labels. In this way, the cell of origin of both intracellular and secreted proteins identified in multicellular culture could be determined. By providing a means to link proteins directly to specific cell types, the authors believe that this new method will be useful in studies of cell-cell communication and biomarker discovery.

Explore further: Brain cancer: Hunger for amino acids makes it more aggressive

More information: Gauthier, N., Sander, C. and Miller, M. Cell-selective labeling using amino acid precursors for proteomic studies of multicellular environments, Nature Methods 2013.

add to favorites email to friend print save as pdf

Related Stories

New technique for IDing proteins secreted by cells developed

Sep 25, 2012

(Phys.org)—Researchers from North Carolina State University have developed a new technique to identify the proteins secreted by a cell. The new approach should help researchers collect precise data on cell biology, which ...

Chemists develop faster, more efficient protein labeling

Feb 05, 2012

North Carolina State University researchers have created specially engineered mammalian cells to provide a new "chemical handle" which will enable researchers to label proteins of interest more efficiently, without disrupting ...

Recommended for you

Researchers successfully clone adult human stem cells

Apr 18, 2014

(Phys.org) —An international team of researchers, led by Robert Lanza, of Advanced Cell Technology, has announced that they have performed the first successful cloning of adult human skin cells into stem ...

Researchers develop new model of cellular movement

Apr 18, 2014

(Phys.org) —Cell movement plays an important role in a host of biological functions from embryonic development to repairing wounded tissue. It also enables cancer cells to break free from their sites of ...

User comments : 0

More news stories

Cancer stem cells linked to drug resistance

Most drugs used to treat lung, breast and pancreatic cancers also promote drug-resistance and ultimately spur tumor growth. Researchers at the University of California, San Diego School of Medicine have discovered ...