Producing protein from circular RNA in E.Coli

May 22, 2013
Producing protein from circular RNA in E.Coli

Circular RNAs were recently shown to be abundant in mice and humans where they influence gene expression.

In a study published today in the journal Angewandte Chemie, the team, led by Dr Hiroshi Abe report that circular treated with the E. coli cell-free system can be translated to produce 100 times more protein than their linear counterparts.

"The translation process is a lot more efficient on circular RNAs than on linear templates because the speed of the re-initiation process is greatly increased," explains Dr. Abe.

This new technique could be used for the synthesis of tandem-repeat peptides such as those found in biologically important proteins such as silk, collagen and epidermal growth factor.

Explore further: Scientists discover mechanistic link between genetic variation and risk of cardiovascular disease

More information: Abe, N. et al. Rolling circle amplification in a prokaryotic translation system using small circular RNA, Angewandte Chemie International Edition, 2013

Related Stories

Circular RNAs more common than previously thought

February 1, 2012

In the classical model of gene expression, the genetic script encoded in our genomes is expressed in each cell in the form of RNA molecules, each consisting of a linear string of chemical "bases". It may be time to revise ...

Recommended for you

Antibody-making bacteria promise drug development

August 31, 2015

Monoclonal antibodies, proteins that bind to and destroy foreign invaders in our bodies, routinely are used as therapeutic agents to fight a wide range of maladies including breast cancer, leukemia, asthma, arthritis, psoriasis, ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.