Minor mutations can lead to big improvements in antibiotic resistance

Jan 09, 2013
Researchers examined the structure and performance of seven forms of a protein called TetX2 that had evolved to allow E. coli to resist attacks by the antibiotic minocycline. This image shows how a molecule of the drug, shown in yellow and gray, fits within the active site of a TetX2 protein. Credit: Y. Shamoo/Rice University

Rice University scientists have found that mutations of small effect can turn out to be game changers in the bacterial fight against antibiotic drugs.

The discovery came during an exhaustive, three-year effort to create a mathematical model that could accurately predict how specific mutations allow bacteria like E. coli to adapt to antibiotics like minocycline. The findings are detailed in a Dec. 10 study in the .

"As biologists, we tend to focus on big effects that result from big changes, but this study shows that bacteria don't have to solve the problem of antibiotic resistance in one giant step," said Rice University Yousif Shamoo, the lead researcher on a new study. "We were surprised to see that small mutations could make a big difference. In some cases, we saw that minor could boost resistance by as much as 500 percent."

Despite the remarkable success of antibiotics, bacterial infections remain a leading cause of death, and antibiotic resistance among bacterial pathogens is a significant threat to public health.

Shamoo, professor of biochemistry and cell biology and director of Rice's Institute of Biosciences and , said the study of antibiotic resistance offers opportunities to both examine complex cellular phenomena and address the "genotype to phenotype problem," one of 's major challenges.

"We've sequenced hundreds of genomes, and we're finding that it's one thing to read the blueprint, and it's another thing to predict how the DNA in that blueprint will affect change in a living cell," Shamoo said.

Shamoo said that because a single antibiotic can make big changes in whether a cell lives or dies, it is possible to make the connection between the gene and the cell's fitness to its environment.

To show that a could accurately predict how specific mutations would affect E. coli resistance, Shamoo's team examined a gene called TetX2. The gene encodes an enzyme that inactivates the antibiotic . The researchers looked at the most likely mutations of the gene and found seven that led to increased . They then spent two years amassing a catalog of basic biochemical measurements for each mutant.

"If this organism were a car, then these enzymatic measurements would be the equivalent of things like the cubic displacement of the engine, the torque and so on," Shamoo said. "These are measurable variables, but by themselves they won't tell you the car's top speed or how fast it can accelerate from 0 to 60 (miles per hour)."

By measuring the performance improvements from each of the seven TetX2 mutants, the group was able to build a mathematical formula that accurately correlated the E. coli resistance with enzyme performance. To test the formula, the team measured the resistance of a new family of mutants and used the formula to calculate their enzyme performance metrics.

"Using the car analogy again, it's like we took one car and measured its top speed and acceleration using seven different engines," Shamoo said. "Then, we took the same car with an unknown engine and showed that we didn't have to open the hood and look to tell what kind of engine it had. We could tell that strictly by knowing its top speed and acceleration." By knowing how the car with the unknown engine performed on the race track, we could describe the engine without ever popping the hood and vice versa.

Shamoo said the research could lead to faster screening methods for resistant strains of bacteria, but the most immediate benefit is an improved understanding of how resistance develops.

"An example of that is this finding about the small steps," he said. "That was unexpected, and it tells us something fundamental about how resistance evolves. We still know that big changes can confer big benefits, but now we know that small changes can yield big benefits too. This tells us that we may need to look at things we might have overlooked in the past."

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More information: www.pnas.org/content/early/2012/12/06/1209335110

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Lino235
1 / 5 (4) Jan 09, 2013
An ancillary conclusion might also be that "major" mutational events are intolerable: i.e., the organism dies. If this is true, then what does this mean for Darwinian mechanisms?
RealScience
not rated yet Jan 09, 2013
@Lino - that conclusion would be wrong.
Some small changes are lethal, some big changes aren't.

Even just within humans, large sections of chromosomes get inverted, deleted or repeated quite frequently.
Some such embryos never develop, while others grow into seemingly normal humans.

Even whole chromosomes can get split, deleted or repeated.
Although that is usually fatal, there are millions of men on earth right now with an entire extra X chromosome, and over 90% of them don't even know it.

That small changes can have big effects supports Darwinian evolution because small changes are extremely common, and hence provide plenty of opportunity for natural selection.

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