Researchers: How are you authenticating your non-human cell lines?

Jan 28, 2013
Left: Mouse. Right: Proof of mouse cell line.

For human cells, researchers have adopted practices that are currently used for human identification, which are based on the detection of short tandem repeat (STR) markers. For the large volume of research that is performed using non-human cell lines (mouse, hamster, monkey, etc.), there are no STR marker assays that are able to uniquely and unequivocally identify a particular cell line.

Researchers found a series of STR markers that uniquely identify and distinguish African green monkey and mouse cell lines. The conditions using multiple labeled primers targeting the STR regions have been optimized, and several widely used cell lines have been genotyped using the assay.

NIST is currently working on an assay for Chinese hamster (CHO) cell authentication and encourages any entity that is interested in kit development to contact us.

Explore further: The origin of the language of life

More information: Almeida, J., Hill, C. and Cole, K. Authentication of African green monkey cell lines using human short tandem repeat markers. BMC Biotechnology 2011, 11:102. www.nist.gov/manuscript-public… ch.cfm?pub_id=908945

add to favorites email to friend print save as pdf

Related Stories

Classifying molar pregnancy

Oct 21, 2009

Researchers from The Johns Hopkins Medical Institutions have used short tandem repeat (STR) genotyping and p57 immunohistochemistry to distinguish hydatidiform moles. The related report by Murphy et al "Molecular Genotyping ...

lobSTR algorithm rolls DNA fingerprinting into 21st century

Apr 27, 2012

As any crime show buff can tell you, DNA evidence identifies a victim's remains, fingers the guilty, and sets the innocent free. But in reality, the processing of forensic DNA evidence takes much longer than a 60-minute primetime ...

New cranial neural crest cell line developed

Sep 19, 2012

Researchers have successfully developed a stable population of neural crest cells derived from mice that can be grown in large quantities in the laboratory and that demonstrates the potential to develop into ...

For stem cells, a way to keep score

Feb 03, 2011

Ever since researchers devised a recipe for turning adult cells into cells that look and act like embryonic stem cells, there has been lingering doubt in the field about just how close to embryonic stem cells each of those ...

Recommended for you

The origin of the language of life

16 hours ago

The genetic code is the universal language of life. It describes how information is encoded in the genetic material and is the same for all organisms from simple bacteria to animals to humans. However, the ...

Quest to unravel mysteries of our gene network

Dec 18, 2014

There are roughly 27,000 genes in the human body, all but a relative few of them connected through an intricate and complex network that plays a dominant role in shaping our physiological structure and functions.

EU court clears stem cell patenting

Dec 18, 2014

A human egg used to produce stem cells but unable to develop into a viable embryo can be patented, the European Court of Justice ruled on Thursday.

User comments : 1

Adjust slider to filter visible comments by rank

Display comments: newest first

Tausch
1 / 5 (1) Jan 28, 2013
The cell lines of life do not always match the genome.
http://en.wikiped...etics%29

Chimeras, from a geneticist's point of view, are living creatures that contain two or more genetically distinct lines of cells that originated from two or more different zygotes. (This is different from another genetic phenomenon called mosaicism, where different cell lines may emerge from one single zygote.) In essence, chimeras are complexes of two genetically different animals.

Scientists have noted long ago the existence of natural chimeras, and these usually occur within the same species.


http://www.scq.ub...himeras/

Is there problem with genetics forensics?
An open question.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.