Researchers: How are you authenticating your non-human cell lines?

Jan 28, 2013
Left: Mouse. Right: Proof of mouse cell line.

For human cells, researchers have adopted practices that are currently used for human identification, which are based on the detection of short tandem repeat (STR) markers. For the large volume of research that is performed using non-human cell lines (mouse, hamster, monkey, etc.), there are no STR marker assays that are able to uniquely and unequivocally identify a particular cell line.

Researchers found a series of STR markers that uniquely identify and distinguish African green monkey and mouse cell lines. The conditions using multiple labeled primers targeting the STR regions have been optimized, and several widely used cell lines have been genotyped using the assay.

NIST is currently working on an assay for Chinese hamster (CHO) cell authentication and encourages any entity that is interested in kit development to contact us.

Explore further: Largest genetic survey to date shows major success of giant panda breeding programs

More information: Almeida, J., Hill, C. and Cole, K. Authentication of African green monkey cell lines using human short tandem repeat markers. BMC Biotechnology 2011, 11:102. www.nist.gov/manuscript-public… ch.cfm?pub_id=908945

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Tausch
1 / 5 (1) Jan 28, 2013
The cell lines of life do not always match the genome.
http://en.wikiped...etics%29

Chimeras, from a geneticist's point of view, are living creatures that contain two or more genetically distinct lines of cells that originated from two or more different zygotes. (This is different from another genetic phenomenon called mosaicism, where different cell lines may emerge from one single zygote.) In essence, chimeras are complexes of two genetically different animals.

Scientists have noted long ago the existence of natural chimeras, and these usually occur within the same species.


http://www.scq.ub...himeras/

Is there problem with genetics forensics?
An open question.