Study reports iron oxide nanoparticles effective for labeling human endothelial cells

Dec 20, 2012

A team of researchers from three medical institutions in Guangzhou, China, have found that iron oxide nanoparticles (INOPS) are a useful contrast agent for in vivo magnetic resonance tracking of transplanted human endothelial cells. However, the impact of INOPS on the cells varies with a number of factors including the INOPS load. They found that the percentage of iron-labeled cells was significantly lower after 48 hours post-transplantation than at 24 hours post-transplantation. They also found that high INOPS concentration can affect cell activity. High INOPS concentration can induce cell death (apoptosis).

Their study is published in the current issue of .

"A good MRI contrast agent must possess a number of features," said study co-author Dr. Wen-Li Chen, at South China Normal University's MOE Key Laboratory of Laser Life Science. "Those features are: low toxicity and good stabilization, high sensitivity, good solubility and the ability to remain in the for a long time. In our study, we found that INOPS are sensitive and can perturb the static magnetic field and provide a string change in MR signals."

The researchers found that the INOPS could be taken up by the cell rather than adhering to the exterior of the cell membrane. However, they also found that a proportional relationship might exist between the number of labeled cells and signal intensity.

"For the first time, we identified that autophagy death could take place at high INOPS loading concentrations," said the researchers.

They also discovered that an increased incubation time, from 24 to 48 hours, did not increase of INOPS and that the percentage of labeled cells declined after 24 hours to be significantly lower by 48 hours.

"It is possible that when the intracellular iron becomes saturated, the particles may start to be expelled out of cells," they concluded. "Thus, the determination of optimal loading concentration is an important step in maintaining the quality of cell labeling and cell activity."

Explore further: Nanocontainers for nanocargo: Delivering genes and proteins for cellular imaging, genetic medicine and cancer therapy

More information: Yang, F-Y.; Yu, M-X.; Zhou, Q.; Chen, W-L.; Gao, P.; Huang, Z. Effects of Iron Oxide Nanoparticle Labeling on Human Endothelial Cells. Cell Transplant. 21(9):1805-1820; 2012.

add to favorites email to friend print save as pdf

Related Stories

MRI contrast agents change stem cell proliferation

Nov 01, 2010

When researchers tested three different labeling agents on three different stem cell populations to determine what effect the labeling agents had on stem cell phenotype, biological behavior and migration abilities, they found ...

Researchers find a better way to track stem cells

Apr 05, 2010

A study published in the current issue of Cell Transplantation (19:1) has found that using the FDA-approved contrast agent Indocyanine Green (ICG) to label human embryonic stem cell-derived cardiomyocytes (hESC-CMs) substa ...

Tiny magnetic particles may help assess heart treatments

Jul 10, 2012

Tiny magnetic particles may help doctors track cells in the body to better determine if treatments work, according to research reported in Circulation: Cardiovascular Imaging, an American Heart Association journal.

Recommended for you

Twisted graphene chills out

Sep 17, 2014

(Phys.org) —When two sheets of graphene are stacked in a special way, it is possible to cool down the graphene with a laser instead of heating it up, University of Manchester researchers have shown.

Researchers use liquid inks to create better solar cells

Sep 17, 2014

(Phys.org) —The basic function of solar cells is to harvest sunlight and turn it into electricity. Thus, it is critically important that the film that collects the light on the surface of the cell is designed ...

User comments : 0