Free-electron lasers reveal detailed architecture of proteins

Jun 01, 2012
Schematic representation of the experimental setup at the Coherent X-ray Imaging endstation at the Linac Coherent Light Source. Millions of tiny crystals are injected into the free-electron laser beam in a thin liquid jet. Diffraction patterns are generated when a crystal intersects a free-electron X-ray flash and are captured on a detector shown on the left.

(Phys.org) -- In the centennial year of Max von Laue’s discovery that X-ray diffraction can be used to unravel the atomic architecture of molecules, a new approach to the determination of high-resolution structures has been demonstrated. An international team of researchers has analyzed tiny protein crystals using short pulses of X-ray light from the world’s first hard X-ray free-electron laser, the US Department of Energy’s 300 million dollar Linac Coherent Light Source at Stanford. The study demonstrates the immense potential of free-electron lasers for obtaining the structures of macromolecules from tiny crystals when illuminated with the blazing intensity of the ultrashort free-electron laser X-ray pulses, even though the crystals are destroyed in the process. In the current study, their structural analysis reveals details with a spatial resolution of 0.2 millionth of a millimeter.

The team, including researchers from the Max Planck Institute for Medical Research in Heidelberg and the Max Planck Advanced Study Group in Hamburg, showed that their data compared well with those collected from large, well-characterized crystals using conventional X-ray sources, providing a benchmark for the new approach. Their proof-of-principle experiment shows that the free-electron laser is an important new tool for structural biology on large macromolecular assemblies and membrane proteins, many of which are known to be important targets for pharmaceutical development.

X-ray free-electron lasers are extremely powerful new X-ray sources that provide highly intense ultrashort flashes of light. The intensity of such an X-ray pulse is more than a billion times higher than that provided by the most brilliant state-of-the-art X-ray sources, with a thousand-fold shorter pulse length, on the order of a few millionths of a billionth of a second, or femtoseconds. These properties provide scientists with novel tools to explore the nano-world, including the structure of biological materials.

Structure of the protein lysozyme. The spatial arrangement of the 129 amino acids is schematically depicted in the form of spirals (helices) and arrows (pleated sheets). © MPI for Medical Research

Most of our knowledge of the three-dimensional spatial architecture of molecules has been obtained by X-ray crystallography, which relies on the amplification of the scattering signal of the molecules by their arrangement into relatively large crystals, often on the order of some tenths of a millimeter. Obtaining large crystals can be extremely difficult in the case of bio-molecules due to their inherent instability and flexibility, as well as their typical low abundance.

Free-electron lasers can obtain structural information from tiny crystals that refuse to reveal their secrets by conventional structural methods due to the damage induced by the radiation used for the structure analysis. Although the tiny crystals are completely destroyed by the incredibly intensity of the free-electron laser, the ultra-short pulses can pass through the sample before the onset of detectable damage and thus provide the necessary scattering signal of the still-intact molecules.

In this diffraction-before-destruction approach, crystals are replenished for serial data collection by injecting them into the free-electron laser beam using a liquid jet, developed by scientists from Arizona State University, exposing one crystal after the other instead of rotating a single large crystal as in conventional crystallography. This concept of serial femtosecond crystallography has been demonstrated before by the same team of researchers at the Linac Coherent Light Source at Stanford, using the CAMP instrument, developed by the Max Planck Advanced Study Group. The relatively long wavelength X-rays then available limited the attainable level of structural detail.

Very recently, a new instrument at the Linac Coherent Light Source, the Coherent X-ray Imaging endstation, has allowed the use of short wavelength X-rays and thus made it possible to infer atomic detail in the molecular architecture. To benchmark the method, a well-characterized model system was investigated, the small lysozyme, the first enzyme ever to have its structure revealed.

The researchers collated ten thousand snapshot exposures from crystals that measured only a thousandth of a millimeter, and showed that the data compared well with those collected using conventional approaches and hundred-fold larger lysozyme crystals. Importantly, no significant signs of radiation damage were detected. “This proof-of-principle experiment shows that the X-ray free-electron laser indeed lives up to its promise as an important new tool for structural biology on large macromolecular assemblies and membrane proteins. It really opens up a completely new terrain in structural biology”, Ilme Schlichting, leading the Max-Planck team, says. Since small crystals are typically easier to produce than large ones, this is of immediate relevance for all studies of molecules that are difficult to crystallize —  as are some 60 percent of all proteins, many of which are prime targets for medical therapies.

Explore further: X-ray powder diffraction beamline at NSLS-II takes first beam and first data

More information: Boutet et al. High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography, Science, June 1, 2012

Related Stories

First X-ray lasing of SACLA

Jun 17, 2011

RIKEN and the Japan Synchrotron Radiation Research Institute (JASRI) have successfully produced a first beam of X-ray laser light with a wavelength of 1.2 Angstroms. This light was created using SACLA, a cutting-edge X-ray ...

Fastest X-ray images of tiny biological crystals

Jan 05, 2012

(PhysOrg.com) -- An international research team headed by DESY scientists from the Center for Free-Electron Laser Science (CFEL) in Hamburg, Germany, has recorded the shortest X-ray exposure of a protein crystal ...

SACLA X-ray free electron laser sets new record

Jun 13, 2011

RIKEN and the Japan Synchrotron Radiation Research Institute (JASRI) have successfully produced a beam of X-ray laser light with a wavelength of 1.2 Angstroms, the shortest ever measured. This record-breaking light was created ...

New research improves quality of free electron laser

Jun 02, 2011

The free electron laser is the next step in the development of equipment to help us see the structure of materials. Nino Čutić at MAX-lab in Lund, Sweden, has done a PhD in further improving the test free electron ...

Recommended for you

Particles, waves and ants

Nov 26, 2014

Animals looking for food or light waves moving through turbid media – astonishing similarities have now been found between completely different phenomena.

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.