Bioluminescent technology for easy tracking of GMO

Apr 30, 2012

It is important to be able to monitor genetically modified (GM) crops, not only in the field but also during the food processing chain. New research published in BioMed Central's open access journal BMC Biotechnology shows that products from genetically modified crops can be identified at low concentration, using bioluminescent real time reporter (BART) technology and loop mediated isothermal amplification (LAMP). The combination of these techniques was able to recognise 0.1% GM contamination of maize, far below the current EU limit of 0.9%.

In agriculture GM crops have been bred to improve crop yield or viability. For example some are resistant to herbicides or viruses. They are also used in the pharmaceutical industry to produce proteins such as collagen. However there is a constant debate about the safety of these crops and whether the man-made transgenes might enter the wild population by cross-fertilization.and produce herbicide .

Careful handling and sampling techniques are required to assess the GM content of a crop. The most common technique is (PCR), however, this involves complex extraction procedures and rapid thermocycling, both of which require specific equipment. To overcome these problems researchers from Lumora Ltd. assessed whether they could use LAMP to amplify DNA at a constant temperature and use BART to identify GM-specific DNA in real time.

Dr Guy Kiddle from Lumora, who led the research, explained that LAMP-BART was able to detect as little as 0.1% GM contamination of maize, and, compared to PCR, was more tolerant of contaminating polysaccharides, meaning that the DNA clean-up process did not need to be as thorough. He commented, "This method requires only basic equipment for , and a constant temperature for and detection. Consequently LAMP-BART provides a 'field-ready' solution for monitoring and their interaction with wild plants or non-GM crops."

Explore further: Scientists see a natural place for 'rewilded' plants in organic farming

Related Stories

What farmers think about GM crops

Feb 24, 2008

Farmers are upbeat about genetically modified crops, according to new research funded by the Economic and Social Research Council (ESRC).

France asks EU to suspend GM crop authorisation

Feb 20, 2012

France's ecology ministry said Monday it had asked European regulators to suspend authorisation for the use of genetically modified MON 810 maize crops from US company Monsanto based on new studies.

Mali farmers don't want GM crops

Jan 31, 2006

Mali farmers say they don't want trials of genetically modified crops to begin in their nation -- the fourth poorest country in the world.

Some GM crops legal in the U.K.

Aug 08, 2005

Genetically modified crops can reportedly be grown in Britain without farmers having to notify the government or their neighbors, the Guardian reported Monday.

Recommended for you

First step towards global attack on potato blight

3 hours ago

European researchers and companies concerned with the potato disease phytophthora will work more closely with parties in other parts of the world. The first move was made during the biennial meeting of the ...

Bacteria study could have agricultural impact

5 hours ago

Wichita State University microbiology professor Mark Schneegurt and ornithology professor Chris Rogers have discovered that one of North America's most common migratory birds – the Dark-eyed Junco – carries ...

Sex chromosomes—why the Y genes matter

16 hours ago

Several genes have been lost from the Y chromosome in humans and other mammals, according to research published in the open access journal Genome Biology. The study shows that essential Y genes are rescue ...

Better mouse model enables colon cancer research

May 27, 2015

Every day, it seems, someone in some lab is "curing cancer." Well, it's easy to kill cancer cells in a lab, but in a human, it's a lot more complicated, which is why nearly all cancer drugs fail clinical ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.