Greater accuracy in gauging blood coagulation

Feb 23, 2012

Thrombin plays a key role in various pathologies of the haemostatic system. Overexpression of thrombin can result in thrombosis, whereas its underexpression might lead to haemophilia. Therefore, accurate monitoring of thrombin activity is crucial for determining the proper treatment of a given patient, as this correlates with the ability of blood to coagulate. A tool for monitoring the activity of thrombin over time is the so-called thrombin generation test (TGT).

In a collaborative project headed by Floris Rutjes (Radboud University Nijmegen, Netherlands), novel thrombin-specific fluorogenic peptides were developed for accurately determining thrombin concentrations by the TGT.

Adding such a peptide substrate to a clotting plasma sample results in its by thrombin, thereby releasing the fluorophore.

Spectrophotometric measurement of fluorophore release increases the sensitivity and thus accuracy of the TGT, yielding various essential coagulation parameters.

The use of fluorogenic peptides in combination with the TGT is expected to find broad application in the field of haemostasis and .

Explore further: Scientists find clues to cancer drug failure

More information: Floris P. J. T. Rutjes, Fluorogenic Peptide-Based Substrates for Monitoring Thrombin Activity, ChemMedChem, dx.doi.org/10.1002/cmdc.201100560

add to favorites email to friend print save as pdf

Related Stories

FDA OKs new adhesive to treat burn victims

Mar 20, 2008

The U.S. Food and Drug Administration approved a medical adhesive -- a fibrin sealant called Artiss -- for use in attaching skin grafts to burn patients.

Blood-clotting protein linked to cancer and septicemia

Feb 04, 2011

In our not-so-distant evolutionary past, stress often meant imminent danger, and the risk of blood loss, so part of our body's stress response is to stock-pile blood-clotting factors. Scientists in the Molecular Medicine ...

Blood clotting and bowel cancer risk

Jun 06, 2011

Back in the mid 19th century, a French doctor, Armand Trousseau, discovered a connection between cancer and thrombosis – the formation of often dangerous blood clots that can lead to venous occlusion. Today it is known ...

Recommended for you

What causes the sunlight flavour in milk?

5 hours ago

Most of us have tasted milk that has been left in the sun – it has a distinctive off-flavour. The reason is that milk and other dairy products turn rancid when exposed to light.

Scientists find clues to cancer drug failure

23 hours ago

Cancer patients fear the possibility that one day their cells might start rendering many different chemotherapy regimens ineffective. This phenomenon, called multidrug resistance, leads to tumors that defy ...

Smart crystallization

Mar 02, 2015

A novel nucleating agent that builds on the concept of molecularly imprinted polymers (MIPs) could allow crystallographers access to proteins and other biological macromolecules that are usually reluctant ...

Supersonic electrons could produce future solar fuel

Mar 02, 2015

Researchers from institutions including Lund University have taken a step closer to producing solar fuel using artificial photosynthesis. In a new study, they have successfully tracked the electrons' rapid transit through ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.