Manipulating genes with hidden TALENs

Feb 13, 2012
X-ray studies carried out at APS and computer modeling of the data reveal how TALEN intimately winds itself into the major groove of target DNA to form a protein-DNA complex just 60 Ĺ x 60 Ĺ x 90 Ĺ. From Amanda Nga-Sze Mak et al., Science 335(6069), 716 (10 February, 2012).

( -- A better understanding of gene function in model plant and animal systems could be used to develop useful traits in livestock and crop plants, and might someday lead to developments in stem cell research and new treatments for human genetic disorders. Those are the projected outcomes of experiments carried out at two Department of Energy (DOE) Office of Science synchrotron x-ray facilities, including the Advanced Photon Source (APS) at Argonne National Laboratory.

A team of researchers from Iowa State University (ISU) and the Fred Hutchinson Cancer Research Center utilized the Life Sciences Collaborative Access Team 21-ID-F x-ray beamline at the APS and beamline 5.0.2 at the Advanced Light Source at the Lawrence Berkeley National Laboratory to take a close look at a group of fused proteins that can control genes. Their work has revealed the crystallographic structure of a protein encoded by an important group of harmful that has evolved to manipulate host gene expression in a specific yet highly adaptable manner.

Xanthomonas bacteria inject TAL effector proteins into the cells of plants that they infect. The proteins wind themselves into the major groove of specific sections of the plants' DNA. This switches-on genes that benefit the invading bacteria. Conversely, the same binding might activate genes that stimulate the plants' defense systems against the pathogens. The step, which involves a molecular recognition process, requires tandem repeat units of the protein building blocks, amino acids, usually 34 long and ending with a half repeat. The repeating patterns have allowed researchers to predict the likely targets for these binding regions and so engineer novel sequences that can also bind to specific strands of DNA.

This research has stimulated renewed interest in the possibility of designing artificial "TAL nuclease" (TALEN) proteins that could target DNA and thereby modify specific genes for a wide variety of purposes in or possibly to even treat human genetic diseases. Adam Bogdanove, of Iowa State University, and colleagues have been building on these discoveries for several years. In 2011, Bogdanove and a team at the University of Minnesota, led by Dan Voytas, formerly of ISU, and a second ISU group led by Bing Yang demonstrated in the laboratory that such a notion was potentially viable as they could create TALENs to manipulate genes and gene functions.

Now, writing in the journal Science in January 2012, Barry Stoddard and colleagues at the Fred Hutchinson Cancer Research Center in Seattle, including post-doc Amanda Mak and faculty member Phil Bradley, have collaborated with Bogdanove and Andres Cernadas to use multiple expression clones and data relating to target site sequences for co-crystallization. The team used a TAL effector, PthXo1, produced by the rice pathogen Xanthomonas oryzae and used x-ray diffraction data collected at the two DOE light sources to solve its three-dimensional (3-D) structure bound to a 36-base pair DNA duplex containing a sequence found in the rice genome itself.

To solve the structure, Bradley developed a high-throughput computational structure prediction method for TAL effectors using the Rosetta software package to generate a highly accurate molecular model for molecular replacement phasing. The resulting structure was then validated against the peaks obtained for selenomethionyl derivative, in which anomalous peaks would match up with the original data only if the model were correct.

Having obtained the 3-D structure of a TAL effector and shown how it physically interacts with double-stranded DNA, the researchers can now figure out the details of the recognition and binding process involved and so get a clearer picture of how they work in nature and how they might be manipulated in the laboratory.

They conclude in their latest Science paper that the work "reveals the hitherto enigmatic structural nature of a simple solution that an important group of pathogens has evolved to manipulate host gene expression in a specific yet highly adaptable manner." Bogdanove describes the structure the team has obtained as "really quite beautiful." "So far there is nothing else in nature quite like it," he said.

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More information: Amanda Nga-Sze Mak, Philip Bradley, Raul A. Cernadas, Adam J. Bogdanove, and Barry L. Stoddard, “The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target,” Science 335(6069), 716 (10 February, 2012).

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