The bigger picture of population genomics

Jan 05, 2012

Not so long ago it was the work of many years to sequence the genome of a single organism: the human genome project, for example, took many laboratories a total of 13 years to complete. The availability of so-called next-generation sequencing methods makes it easy – and comparatively cheap – to sequence DNA, although sequencing the large number of individuals required for population genetics studies is still time-consuming and costly and has thus been restricted to few organisms.

The group of Christian Schlötterer of the Institute of Genetics at the University of Veterinary Medicine, Vienna has shown previously that pooling samples enables studies to be undertaken at significantly reduced costs. Despite the wide applicability and obvious power of the method, however, it has so far proven possible to apply next-generation sequencing at the scale of populations to only few model systems. The problem lies in the interpretation of the data. And this is where the latest work from Schlötterer's group comes in. Robert Kofler, Ram Vinay Pandey and Schlötterer now report the development of a software package – catchily termed "PoPoolation2" – that makes it possible even for non-experts to compare populations.

The package offers a wide range of statistical methods to determine how the frequencies of particular forms – termed alleles – of genes vary between populations. The program has been tested on the sequences of a single chromosome from two distinct populations of the fruit fly Drosophila melanogaster and the results confirm that the program can correctly predict the levels of divergence between the samples. As Schlötterer says, "PoPoolation2 helps us compare the allele frequencies between populations. It will enable us quickly and cheaply to compare how populations of different species have adapted differently to their environments, giving us better information on the big picture of evolution in practice."

Explore further: How does enzymatic pretreatment affect the nanostructure and reaction space of lignocellulosic biomass?

More information: The paper 'PoPoolation2: Identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq)' by Robert Kofler, Ram Vinay Pandey and Christian Schlötterer is published in the current issue of the journal Bioinformatics and is available online at bioinformatics.oxfordjournals.… tics.btr589.full.pdf

Provided by University of Veterinary Medicine -- Vienna

not rated yet
add to favorites email to friend print save as pdf

Related Stories

Researchers discover new bone deformity gene

May 04, 2011

(Medical Xpress) -- The Human Genetics team at The University of Queensland Diamantina Institute have successfully used a new gene-mapping approach for patients affected by severe skeletal abnormalities.

Recommended for you

Quest to unravel mysteries of our gene network

12 hours ago

There are roughly 27,000 genes in the human body, all but a relative few of them connected through an intricate and complex network that plays a dominant role in shaping our physiological structure and functions.

EU court clears stem cell patenting

13 hours ago

A human egg used to produce stem cells but unable to develop into a viable embryo can be patented, the European Court of Justice ruled on Thursday.

User comments : 1

Adjust slider to filter visible comments by rank

Display comments: newest first

winthrom
not rated yet Jan 05, 2012
This also allows comparison of people populations: e.g., populations of Scottish Europian men with Spanish Europian men. Any number of paired population variations may be compared, say centenarian families and non-centenarian families. Eventually a library of variants in DNA may be identified with a description as to potential heriditary consequences. We are well on our way towards modifying genes within living organisims (adult stem cell work) so we may be approaching tailored genomic medicine also.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.