Fast new method for mapping blood vessels may aid cancer research

In a paper published in the October issue of the Optical Society's open-access journal Biomedical Optics Express, computational neuroscientists at Texas A&M University, along with collaborators at the University of Illinois and Kettering University, describe a new system, tested in mouse brain samples, that substantially reduces that time.

The method uses a technique called knife-edge scanning microscopy (KESM). First, blood vessels are filled with ink, and the whole brain sample is embedded in plastic. Next, the plastic block is placed onto an automated vertically moving stage. A diamond knife shaves a very thin slice – one micrometer or less – off the top of the block, imaging the sample line by line at the tip of the knife.

Enlarge

Reconstruction of a small section from the previous image, showing the relative thickness of each blood vessel in the network (color-coded by thickness). The area depicted in the image is about 0.275 millimeters across. Credit: Biomedical Optics Express

Each tiny movement of the stage triggers the camera to take a picture. In this way, the researchers can get the full 3-D structure of the mouse brain's vascular network – from arteries and veins down to the smallest capillaries – in less than two days at full production speed. In the future the team plans to augment the process with fluorescence imaging, which will allow researchers to link brain structure to function.

More information: "Fast macro-scale transmission imaging of microvascular networks using KESM," Biomedical Optics Express, Mayerich et al., Vol. 2, Issue 10, pp. 2888-2896 (2011).

Provided by Optical Society of America