Long-standing plant biochemistry mystery solved

Sep 19, 2011
Models of the complete desaturase-substrate complexes: A shows the castor desaturase (grey “ribbon”) with the fatty acid (light blue “stick” molecule) attached to ACP (dark blue). The aspartic amino acid is highlighted in yellow. B shows the ivy desaturase (grey) with the fatty acid (teal “stick” molecule) attached to ACP (green), and the amino acid lysine highlighted in yellow. In both figures, the shaded area around the fatty acid represents the surface of the substrate-binding cavity. The diagrams show that substituting lysine for aspartic acid changes the binding configuration, allowing the fatty acid chain to further penetrate the binding cavity. Thus this single amino-acid switch results in desaturation at a different site.

Scientists at the U.S. Department of Energy's (DOE) Brookhaven National Laboratory and collaborators at the Karolinska Institute in Sweden have discovered how an enzyme "knows" where to insert a double bond when desaturating plant fatty acids. Understanding the mechanism -- which relies on a single amino acid far from the enzyme's active site -- solves a 40-year mystery of how these enzymes exert such location-specific control. The work, published in the Proceedings of the National Academy of Sciences the week of September 19, 2011, may lead to new ways to engineer plant oils as a renewable replacement for petrochemicals.

"Plant are an approximately $150-billion-dollar-a-year market," said Brookhaven John Shanklin, lead author on the paper. "Their properties, and therefore their potential uses and values, are determined by the position of double bonds in the hydrocarbon chains that make up their backbones. Thus the ability to control double bond positions would enable us to make new designer fatty acids that would be useful as industrial raw materials."

The enzymes responsible for double-bond placement, called desaturases, remove and insert between adjacent at specific locations on the hydrocarbon chains. But how one enzyme knows to insert the double bond at one location while a different but closely related enzyme inserts a double bond at a different site has been a mystery.

"Most enzymes recognize features in the molecules they act on that are very close to the site where the enzyme's action takes place. But all the carbon-hydrogen groups that make up fatty-acid backbones are very similar with no distinguishing features — it's like a greasy rope with nothing to hold onto," said Shanklin.

In describing his group's long-standing quest to solve the desaturation puzzle, Shanklin quotes Nobel laureate Konrad Bloch, who observed more than 40 years ago that such site-specific removal of hydrogen "would seem to approach the limits of the discriminatory power of enzymes."

Shanklin and his collaborators approached the problem by studying two genetically similar desaturases that act at different locations: a castor desaturase that inserts a double bond between carbon atoms 9 and 10 in the chain (a 'delta-9' desaturase); and an ivy desaturase that inserts a double bond between carbon atoms 4 and 5 (delta-4). They reasoned that any differences would be easy to spot in such extreme examples.

But early attempts to find a telltale explanation — which included detailed analyses of the two enzymes' atomic-level crystal structures — turned up few clues. "The crystal structures are almost identical," Shanklin said.

The next step was to look at how the two enzymes bind to their substrates — fatty acid chains attached to a small . First the scientists analyzed the crystal structure of the castor desaturase bound to the substrate. Then they used computer modeling to further explore how the carrier protein "docked" with the enzyme.

"Results of the computational docking model exactly matched that of the real crystal structure, which allows carbon atoms 9 and 10 to be positioned right at the enzyme's active site," Shanklin said.

Next the scientists modeled how the carrier protein docked with the ivy desaturase. This time it docked in a different orientation that positioned carbon atoms 4 and 5 at the desaturation active site. "So the docking model predicted a different orientation that exactly accounted for the specificity," Shanklin said.

To identify exactly what was responsible for the difference in binding, the scientists then looked at the amino acid sequence — the series of 360 building blocks that makes up each enzyme. They identified amino acid locations that differ between delta-9 and delta-4 desaturases, and focused on those locations that would be able to interact with the substrate, based on their positions in the structural models.

The scientists identified one position, far from the active site, where the computer model indicated that switching a single amino acid would change the orientation of the bound fatty acid with respect to the active site. Could this distant amino-acid location remotely control the site of double bond placement?

To test this hypothesis, the scientists engineered a new desaturase, swapping out the aspartic acid normally found at that location in the delta-9 castor desaturase for the lysine found in the delta-4 ivy desaturase. The result: an enzyme that was castor-like in every way, except that it now seemed able to desaturate the fatty acid at the delta-4 carbon location. "It's quite remarkable to see that changing just one amino acid could have such a striking effect," Shanklin said.

The computational modeling helped explain why: It showed that the negatively charged aspartic acid in the castor desaturase ordinarily repels a negatively charged region on the carrier protein, which leads to a binding orientation that favors delta-9 desaturation; substitution with positively charged lysine results in attraction between the desaturase and carrier protein, leading to an orientation that favors delta-4 desaturation.

Understanding this mechanism led Ed Whittle, a research associate in Shanklin's lab, to add a second positive charge to the castor desaturase in an attempt to further strengthen the attraction. The result was a nearly complete switch in the castor enzyme from delta-9 to delta-4 desaturation, adding compelling support for the remote control hypothesis.

"I really admire Ed's persistence and insight in taking what was already a striking result and pushing it even further to completely change the way this enzyme functions," Shanklin said.

"It's very rewarding to have finally solved this mystery, which would not have been possible without a team effort drawing on our diverse expertise in biochemistry, genetics, computational modeling, and x-ray crystallography.

"Using what we've now learned, I am optimistic we can redesign enzymes to achieve new desirable specificities to produce novel fatty acids in plants. These novel fatty acids would be a renewable resource to replace raw materials now derived from petroleum for making industrial products like plastics," Shanklin said.

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jonnyboy
1 / 5 (1) Sep 19, 2011
I just love real science.
CHollman82
2.3 / 5 (3) Sep 19, 2011
Very cool how little things like proteins and enzymes react automatically in a way controlled by nothing but the laws of physics to produce such incredible complexity. I understand why creationists mistakenly believe that the complexity they see must have been designed, but once you understand stuff like this you realize the truth is even more amazing than the fiction.
epsi00
not rated yet Sep 20, 2011
An enzyme, with no brain, knows what to do and where over and over yet many people ( scientists who study animals among them ) are surprised that animals, with brain, can think! or are self-conscious.
kevinrtrs
1 / 5 (5) Sep 20, 2011
@CHollman82. It's even more amazing when you get to think about where the biological enzymes and proteins we see in life forms come from. And that they can in no wise arise automatically from random chemical reactions, at least not to the extent that they can perform the actions required for life.

You really have to deceive yourself mightily in order to believe that the complexity you see just arose all by itself.

First you have the chemical complexity just by itself. Then you have a 3-dimensional physical geometry to contend with - which is of course closely linked to your chemical bonds. There is nothing automatic about it until it has all been put in place by some intelligent mind first.
Why do you think it has taken up to now for someone to actually figure these bonds out programmatically so that it will now enable them to design proteins and enzymes digitally before easily constructing them?

Ask yourself this question: where did life come from? What answer do you get?
hush1
not rated yet Sep 20, 2011
@kevinrers
From zero. The answer.

Something from nothing:
The consistency starts with the expression; '0 = God '

Your consistency starts with God.
My consistency starts with zero.

Your consistency excludes zero.
My consistency includes God.

Your answer is inconsistent.
My answer is consistent.
CHollman82
1 / 5 (1) Sep 21, 2011
@CHollman82. It's even more amazing when you get to think about where the biological enzymes and proteins we see in life forms come from. And that they can in no wise [sic] arise automatically from random chemical reactions, at least not to the extent that they can perform the actions required for life.


Except that is exactly what we do see... all of our observations over the last few hundred years disagree with what you're saying here.

You really have to deceive yourself mightily in order to believe that the complexity you see just arose all by itself.


Yet every single observation we have made leads us to that conclusion... What I believe is based on evidence, what you believe is based on ignorance and superstition.