Mass spectrometry and imaging facilities enable cancer cell discovery

Aug 19, 2011

A breakthrough in the laboratory of Kevin Vaughan, associate professor of biological sciences at the University of Notre Dame, will assist researchers in understanding cell cycle regulation. The group identified a novel protein that is regulated by the mitotic kinase Aurora B, an important factor in mitosis, or cell division.

In addition to cancers with a genetic origin, such as colon and breast cancers, mistakes in are considered a leading cause of spontaneous cancers.

The research was published this month in Molecular Biology of the Cell, the official journal of the American Society for Cell Biology. The discovery was made possible by collaboration with the Notre Dame Integrated Imaging Facility and the Notre Dame and Proteomics Facility.

Graduate students James Kasuboski and Jason Bader in Vaughan’s lab conducted most of the study, collaborating with Bradley Smith, director of the Integrated Imaging Facility, and William Boggess, director of the Mass Spectrometry and Proteomics Facility. Using cutting-edge equipment, the researchers were able to visualize chromosomes during the process of alignment and to detect errors in alignment when Aurora B activity was abnormal. New instruments in the mass spectrometry and proteomics facility allowed the investigators to identify the substrate for B kinase, a protein critical to chromosome signaling during this process.

The published research identifies this novel protein and its role in coupling the process of alignment with accurate chromosome segregation. Undergraduate biology majors Meghan Morrissey and Michael Winding also contributed to the project.

Vaughan, who is on the steering committee of both facilities, says the combination gave a critical advantage to the Notre Dame researchers compared to those who have access to only one of the technologies. The facilities became fully functional about 18 months ago, and the research is one of the first to demonstrate the power of the facilities.

“Other scientists don’t have access to these sophisticated instruments, which makes this type of work very difficult,” Vaughan says, adding that acquisition of the facilities started three years ago with funds from the Office of the Provost to support research infrastructure. “We coupled imaging with mass spectrometry, both in state-of-the-art facilities. It’s an example of what can happen with other projects.”

Explore further: Students use physics to unpack DNA, one molecule at a time

add to favorites email to friend print save as pdf

Related Stories

Argonne, Notre Dame begin new nuclear theory initiative

Oct 05, 2005

Physicists at the U.S. Department of Energy's Argonne National Laboratory and the University of Notre Dame have begun a new collaborative project to explore and explain the physics of rare nuclear isotopes.

Recommended for you

Brand new technology detects probiotic organisms in food

Jul 23, 2014

In the food industr, ity is very important to ensure the quality and safety of products consumed by the population to improve their properties and reduce foodborne illness. Therefore, a team of Mexican researchers ...

Protein evolution follows a modular principle

Jul 23, 2014

Proteins impart shape and stability to cells, drive metabolic processes and transmit signals. To perform these manifold tasks, they fold into complex three-dimensional shapes. Scientists at the Max Planck ...

Report on viruses looks beyond disease

Jul 22, 2014

In contrast to their negative reputation as disease causing agents, some viruses can perform crucial biological and evolutionary functions that help to shape the world we live in today, according to a new report by the American ...

User comments : 0