Researchers extend genetic code of an entire animal

Aug 15, 2011 by Bob Yirka report
Caenorhabditis elegans. Image: Wikipedia.

(PhysOrg.com) -- Researchers Sebastian Greiss and Jason Chin of the Medical Research Council's Laboratory of Molecular Biology in Cambridge, have succeeded in manipulating the DNA of a nematode such that a 21st protein was created and subsequently naturally replicated throughout the entire worm. The result, which the two describe in their paper in the Journal of the American Chemical Society, is the first ever animal with artificial information embedded in its genetic code.

The protein created glows bright red when exposed to UV light, causing all 1000 cells in the 1 mm long Caenorhabditis elegans to glow like a string of Christmas lights under the microscope, proving that the procedure worked.

Normally there are just 20 amino acids used by all ; in this case though, the two researchers have altered the DNA of the nematode (which details how the amino acids should assemble themselves to make proteins) so that it creates a 21st. Previously such a feat had only been accomplished with a simple , (at the Scripps Institute ten years ago where Dr. Chin was part of another team). The technique works by manipulating one of the A, C, G or T DNA letters such that cells reading it would interpret it as a guide for producing the previously unknown amino acid.

The results demonstrated are considered to be a transformative event in microbiological science because it suggests that it will now be possible to create a whole host of new amino acids and proteins in animals, each limited only by the imagination. Initially it is expected that such proteins will be of the kind that would be sensitive or reactive to light, thus allowing researchers to cause or control effects in an animal by doing nothing more than illuminating them. Such experiments would be truly groundbreaking because unlike previous trials that sought to recreate natural phenomenon, new research in this area could lead to the creation of new types of animal biology.

Greiss and Chin next plan to work together to see if a means can be found to control neural cells in the nematode brain that would lead to directing animal behavior using simple laser flashes.

Explore further: The origin of the language of life

More information: Sebastian Greiss and Jason W Chin, Expanding the Genetic Code of an Animal, J. Am. Chem. Soc., Just Accepted Manuscript, DOI: 10.1021/ja2054034

ABSTRACT
Genetic code expansion, for the site-specific incorporation of unnatural amino acids into proteins, is currently limited to cultured cells and unicellular organisms. Here we expand the genetic code of a multicellular animal, the nematode Caenorhabditis elegans (C. elegans).

Related Stories

Research breakthrough for the protein factories of tomorrow

Sep 22, 2006

Using a kind of molecular ‘hip joint operation,’ researchers at Uppsala University have succeeded in replacing a natural amino acid in a protein with an artificial one. This step forward opens the possibility of creating ...

'Fishy' clue helps establish how proteins evolve

Jan 27, 2009

(PhysOrg.com) -- Three billion years ago, a "new" amino acid was added to the alphabet of 20 that commonly make up proteins in organisms today. Now researchers at Yale and the University of Tokyo have demonstrated ...

Recommended for you

The origin of the language of life

Dec 19, 2014

The genetic code is the universal language of life. It describes how information is encoded in the genetic material and is the same for all organisms from simple bacteria to animals to humans. However, the ...

Quest to unravel mysteries of our gene network

Dec 18, 2014

There are roughly 27,000 genes in the human body, all but a relative few of them connected through an intricate and complex network that plays a dominant role in shaping our physiological structure and functions.

EU court clears stem cell patenting

Dec 18, 2014

A human egg used to produce stem cells but unable to develop into a viable embryo can be patented, the European Court of Justice ruled on Thursday.

User comments : 7

Adjust slider to filter visible comments by rank

Display comments: newest first

Techno1
3.6 / 5 (5) Aug 15, 2011
Great.

Now make a plant species that can grow chicken breast meat without the poop and feathers!!

This is freaky.

We could be looking at a complete revolution in manufacturing and refining of SOME chemicals as well.
knowledge_treehouse
not rated yet Aug 15, 2011
This was done on a mature organism?
Techno1
1 / 5 (4) Aug 15, 2011
This was done on a mature organism?


No way. That would require modifying every individual cell, which would not only be impossible, but would take centuries even if you could do it, even for a microscopic organism..

They must have modified a gamete or used a virus or something like that.
antialias_physorg
5 / 5 (2) Aug 15, 2011
They used something called 'biolistic bombardment' (which can be looked up on wikipedia under the heading of 'gene gun')
http://en.wikiped...Gene_gun
Then selectively killing the worms that did not express the genes and breeding the rest.

At least that what I got from the paper (I'm no biochemist so this analysis may be wrong. The paper is a veritable cornucopia of technical terms I'm not familiar with).

The paper is available from the link in the article - though you have to register with ACS (which is free and took me all of 3 minutes to do)
epsi00
3.8 / 5 (4) Aug 15, 2011
It would be interesting to add a "glow in red" amino acid to politicians when they lie. Imagine how it would look when congress is debating something.
Scottingham
not rated yet Aug 15, 2011
Excellent, another step towards true genetic engineering, versus genetic kludging.
Moebius
3.3 / 5 (4) Aug 15, 2011
It would be interesting to add a "glow in red" amino acid to politicians when they lie.


Glow in red? I'd rather see it cause nausea and vomiting with an ever increasing chance of terminal cancer. Downside is every seat in government would need a barf bag.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.