Cooling system may build eggs' natural defenses against salmonella

Jun 21, 2011 by Brian Wallheimer
Kevin Keener's research has shown that cooling eggs after they are laid may increase the natural defenses those eggs have against bacteria such as Salmonella. Credit: Purdue Agricultural Communication file photo/Tom Campbell

(PhysOrg.com) -- Once eggs are laid, their natural resistance to pathogens begins to wear down, but a Purdue University scientist believes he knows how to rearm those defenses.

Kevin Keener, an associate professor of food science, created a process for rapidly cooling eggs that is designed to inhibit the growth of bacteria such as salmonella. The same cooling process would saturate the inside of an egg with carbon dioxide and alter , which he has found are connected to the activity of an enzyme called lysozyme, which defends egg whites from bacteria.

"This enzyme activity is directly related to the carbon dioxide and pH levels," said Keener, whose results were published in the journal Poultry Science. "An increase in lysozyme would lead to increased safety in eggs."

Freshly laid eggs are saturated with carbon dioxide and have pH levels of about 7. Over time, the rises to 9 and carbon dioxide escapes, Keener said. As that happens, lysozyme becomes less active.

Keener saturated purified egg white lysozymes with carbon dioxide and tested different pH levels. He found that at both high and low pH levels, the addition of carbon dioxide would increase lysozyme activity by as much as 50 percent.

The cooling process Keener developed would create the same conditions, he said.

"When we cool the eggs, carbon dioxide is sucked inside the shell," Keener said. "We're able to resaturate the white of the egg with carbon dioxide, returning it to that original condition when the chicken laid it."

The additional lysozyme activity would give eggs more time to self-eliminate .

Keener's cooling technology uses carbon dioxide "snow" to rapidly lower the eggs' temperature. Eggs are placed in a cooling chamber and at about minus 110 degrees Fahrenheit is generated. The is circulated around the eggs and forms a thin layer of ice inside the eggshell. After treatment, the ice layer melts and quickly lowers an egg's internal temperature to below 45 . The eggshell does not crack during this process because it can resist expansion from a thin ice layer.

Keener said Food and Drug Administration studies show that if eggs were cooled and stored at 45 degrees or less within 12 hours of laying, there would be an estimated 100,000 fewer salmonella illnesses from eggs in the United States each year.

Keener will continue to study the molecular changes that occur with egg cooling.

Explore further: New research finds that world-class sprinters attack the ground to maximize impact forces and speed

More information: Influence of Carbon Dioxide on the Activity of Chicken Egg White Lysozyme, P. Banerjee, K.M. Keener and V.D. Lukito, Poultry Science.

add to favorites email to friend print save as pdf

Related Stories

Scrambling for climate change solutions

Oct 26, 2010

The food industry generates a lot of waste products, but one of these, eggshells, could help combat climate change, according to research published in the International Journal of Global Warming this month.

Scientists discuss carbon dioxide uses

Mar 13, 2006

The use of environmentally friendly carbon dioxide as a refrigerant instead of ozone-depleting chemicals will be discussed at Purdue University this week.

Robot with frog egg smell sensor (w/ Video)

Aug 26, 2010

(PhysOrg.com) -- Researchers from the University of Tokyo have invented a novel means of improving a robot's sense of smell, by using inexpensive olfactory sensors containing frog eggs.

Recommended for you

The roots of human altruism

26 minutes ago

Scientists have long been searching for the factor that determines why humans often behave so selflessly. It was known that humans share this tendency with species of small Latin American primates of the ...

The devastating spread of the mountain pine beetle

54 minutes ago

When the mountain pine beetle began blazing a path across forests in British Columbia and Alberta, nobody could have imagined the extent of the damage to come. But as the insect devastated pine forests and ...

Researchers film protein quake for the first time

1 hour ago

One of nature's mysteries is how plants survive impact by the huge amounts of energy contained in the sun's rays, while using this energy for photosynthesis. The hypothesis is that the light-absorbing proteins ...

User comments : 0