Team shapes cell behavior research

Mar 10, 2011

A team led by James Henderson, assistant professor of biomedical and chemical engineering in Syracuse University's L.C. Smith College of Engineering and Computer Science (LCS) and researcher in the Syracuse Biomaterials Institute, has used shape memory polymers to provide greater insight into how cells sense and respond to their physical environment.

Most cell biomechanics research has examined on unchanging, flat surfaces. "Living cells are remarkably complex, dynamic and versatile systems, but the material substrates currently used to culture them are not," says Henderson (at right in photo). "What motivated our work was the need for cell culture technologies that would allow dynamic control of cell-material interactions. We wanted to give a powerful new tool to biologists and bioengineers."

The goal of the current research was to develop a temperature-sensitive polymer substrate that could be programmed to change shape under cell-compatible conditions. Shape memory polymers (SMPs) are a class of "smart" materials that can switch between two shapes on command, from a fixed (temporary) shape to a pre-determined permanent shape, via a trigger such as a temperature change.

The breakthrough needed to achieve the research goal was made by Kevin Davis, a third-year Ph.D. student in the Henderson lab. Davis was able to develop a SMP with a that worked within the limited range required for cells to live. He observed greater than 95 percent cell viability before and after topography and temperature change. This is the first demonstration of this type of cell-compatible, programmable topography change. Davis' and Henderson's work collaboration with Kelly Burke of Case Western Reserve University and Patrick T. Mather, Milton and Ann Stevenson Professor of Biomedical and Chemical Engineering at Syracuse University, is highlighted in the January issue of the journal Biomaterials, the leading journal in biomaterials research.

After confirming that cells remained viable on the substrate, Davis then investigated the changes in cell alignment on the surface that results from topography change. Davis programmed a SMP substrate that transitioned from a micron-scale grooved surface to a smooth surface. When the cells were seeded on the grooved sample at 30ºC, the cells lined up along the grooves of the surface. The substrates were then placed in a 37ºC incubator, which was the transition temperature for the substrate to recover to a smooth surface. Following shape memory recovery, the cells were observed to be randomly oriented on the substrate.

This research project aimed to determine if cells could remain viable with a change in substrate topography and determine whether cells responded to the change. The next phase of this research is to move from a 2D substrate to a 3D substrate and examine cell viability. Additionally, Henderson's team will be looking at what is going on inside the cells as a result of topography changes.

The application of shape memory principles offers potential solutions for current limitations of static substrate research in bioengineering research, such as medical devices and tissue engineering scaffolds. "For the first time, we've shown that this general concept can be used successfully with , which suggests that it can be extended to a number of biomaterials that could be used for scaffolds and many other applications," says Davis. Since most scaffolding is made out of polymers, Henderson envisions one day using SMPs to create scaffolds that can expand inside the body, allowing for less invasive surgical procedures.

Explore further: Two teams pave way for advances in 2D materials

Provided by Syracuse University

not rated yet

Related Stories

Functionally graded shape memory polymers developed

Jan 05, 2011

(PhysOrg.com) -- A team led by Patrick T. Mather, director of Syracuse Biomaterials Institute (SBI) and Milton and Ann Stevenson professor of biomedical and chemical engineering in Syracuse University’s ...

New process promises to revolutionize manufacturing of products

Sep 01, 2010

A new "smart materials" process - Multiple Memory Material Technology - developed by University of Waterloo engineering researchers promises to revolutionize the manufacture of diverse products such as medical devices, microelectromechanical ...

Polymer remembers four shapes

Mar 15, 2010

(PhysOrg.com) -- A new study by General Motors has found that a polymer used commercially in fuel cell membranes can "memorize" four shapes, each assigned to a different temperature. The material could find ...

Finding the right mix: A biomaterial blend library

Oct 27, 2006

From dental implants to hip replacements, biomaterials have become big business. But scientists pursuing this modern medical revolution share a basic challenge: biocompatibility. How will a biomaterial on the ...

Stem cells shape up to their surroundings

Oct 07, 2010

Many scientists aspire to take control over the stem cell differentiation process, so that we can grow organs and implants perfectly matched to each patient in the future. Now research in the Journal of Tissue Engineering explai ...

Recommended for you

Scientists develop pioneering new spray-on solar cells

13 hours ago

(Phys.org) —A team of scientists at the University of Sheffield are the first to fabricate perovskite solar cells using a spray-painting process – a discovery that could help cut the cost of solar electricity.

User comments : 0