Caffeine energizes cells, boosting virus production for gene therapy applications

Jan 25, 2011
Human Gene Therapy is published monthly in print and online by Mary Ann Liebert Inc., publishers. Credit: © 2010, Mary Ann Liebert Inc., publishers

Give caffeine to cells engineered to produce viruses used for gene therapy and the cells can generate 3- to 8-times more virus, according to a paper published in Human Gene Therapy, a peer-reviewed journal published by Mary Ann Liebert, Inc.

This simple and inexpensive strategy for increasing lentivirus production was developed by Brian Ellis, Patrick Ryan Potts, and Matthew Porteus, University of Texas Southwestern Medical Center, Dallas. In their paper, "Creating Higher Titer Lentivirus with Caffeine," they emphasize that the timing of caffeine addition to standard lentiviral production protocols is important for achieving higher virus titers. Caffeine concentration is also critical, as too much caffeine was toxic to the and did not increase .

Lentivirus vectors are commonly used for transferring genes into cells for both research applications in the laboratory and, increasingly, for procedures in clinical testing. The addition of "should significantly decrease the cost of lentiviral production for research and clinical uses," conclude the authors.

"It is ironic that the ingredient in beverages like colas and coffees that helps keep us awake and alert is also useful in jazzing up cells to produce more gene therapy vectors. An increase in vector production of 5-fold may prove critical in establishing the commercial viability of lentiviral based products," says James M. Wilson, MD, PhD, Editor-in-Chief, and Director of the Gene Therapy Program, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia.

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More information: The paper is available free online at www.liebertpub.com/hum

Provided by Mary Ann Liebert, Inc.

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User comments : 5

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gmurphy
4.7 / 5 (3) Jan 25, 2011
Caffeine can also be used to make programmers produce more code, coincidence?, I think not :)
that_guy
1 / 5 (1) Jan 25, 2011
So...does this translate to someone who drinks caffeine? I'm not in the mood to stop drinking my morning coffee, but I don't like the idea that I might produce 3-8 times more viruses.
Lordjavathe3rd
5 / 5 (1) Jan 25, 2011
Are they sure the caffeine doesn't just make the cells "feel" more productive?
MLSASCP
not rated yet Jan 26, 2011
Since caffeine has a relatively short half life (around 6 hrs) in the peripheral bloodstream my guess is that drinking it will not effect your own "viral load."
that_guy
1 / 5 (1) Jan 28, 2011
Are they sure the caffeine doesn't just make the cells "feel" more productive?


LOL

@MLSASCP
Since caffeine has a relatively short half life (around 6 hrs) in the peripheral bloodstream my guess is that drinking it will not effect your own "viral load."


Regardless of any merit to the question, first and foremost, my first comment was a joke...

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