One-touch make-up -- for our cells (w/ Video)

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Instead of spending hours applying first one colour of make-up – or fluorescent label – and then another, scientists were able to create the equivalent of a make-up brush that is applied only once and highlights different features simultaneously.

The underlying technique was first developed by Imre Berger from the European Molecular Biology Laboratory (EMBL) in Grenoble, France, as part of a method called MultiBac, for expressing protein complexes in insect cells.

In work published today in Nature Communications, Imre Berger and Philipp Berger from the Paul Scherrer Institut (PSI) in Villigen, Switzerland, joined forces to adapt this technology concept to mammalian cells like our own for the first time. It essentially involves rapidly engineering a single vector to deliver a theoretically unlimited number of foreign genes to a cell. To date, the scientists have successfully delivered up to 15 genes in this way.

The protein encoded by each of those genes can carry a fluorescent label, so this makes multiple labelling much more efficient than previous methods. The new labelling technique for mammalian cells, called MultiLabel, could help make drug development and screening considerably faster, since it allows scientists to precisely label many cellular components involved in a given disease process and follow them all at the same time.

More information: Kriz, A., Schmid, K., Baumgartner, N., Ziegler, U., Berger, I., Ballmer-Hofer, K. & Berger, P. A plasmid-based multigene expression system for mammalian cells. Nature Communications, Advanced Online Publication 16 November 2010. DOI:10.1038/ncomms1120

Provided by European Molecular Biology Laboratory