NRL scientists unravel complex quantum dot-dopamine interactions

Oct 15, 2010
Peptides conjugated to dopamine are pre-reduced to hydroquinone and then self-assembled to QDs. At low pH, hydroquinone is predominant and as a poor electron acceptor this results in low QD photoluminescence (PL) quenching. As pH increases, ambient O2 in the buffer oxidizes dopamine producing a hydrogen peroxide species. The increasing quinone concentration provides favorable electron acceptors in close proximity to the QD. This produces higher quenching efficiencies with a magnitude directly proportional to the amount of quinone and hence pH. Credit: Naval Research Laboratory/Scripps Research Institute

Scientists at the Naval Research Laboratory (NRL) in conjunction with the Scripps Research Institute in La Jolla, Ca., recently reported a detailed study of the interactions of water soluble semi-conductor quantum dots (QDs) with the electro-active neuro-transmitter dopamine.

These biocompatible QD-dopamine nano-assemblies may be used as the active component for sensors that are used to detect a wide variety of target analytes ranging from sugars to peroxides.

According to NRL's Dr. Michael Stewart, a member of the research team "The nature of the QD-dopamine interaction has been the subject of more than 25 recent research papers that attempted to uncover and exploit the exact nature of how the QDs interact with these small electro-active chemicals during the sensing process. Until now, it remained unclear as to whether dopamine acted as an electron acceptor or as an electron to quench luminescence from the QD."

Fluorescent micrographs collected from COS-1 cells co-injected with 550 nm emitting QD-dopamine conjugates and red FLX internal standard nanospheres in buffer at pH 6.5. The growth media was switched to pH 11.5 supplemented with the drug Nystatin and micrographs were captured at the indicated time intervals from both the QD and FLX emission channels. Merged images are shown in the bottom row and pH values extracted at each time interval are shown below. Credit: Naval Research Laboratory/Scripps Research Institute

"The chemical state of changes from a protonated hydroquinone in acidic media to an oxidized quinone in basic environments. A series of carefully designed experiments allowed the research team to establish that only the quinone form is capable of acting as an electron acceptor resulting in quenching of the QD emission. The rate of quinone formation and hence QD quenching is directly proportional to pH and can therefore be used to detect changes in the pH of solutions. Using this nano-scale sensor, the research team was able to demonstrate pH sensing in solution and even visualize changes inside cells as underwent drug-induced alkalosis," explained Dr. Scott Trammell.

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More information: The research was published in the August 2010 issue of Nature Materials.

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