Cord blood-derived CD133+ cells improve cardiac function after myocardial infarction

Feb 03, 2010

Researchers at the Pontifícia Universidade Católica do Paraná and Instituto Carlos Chagas have evaluated the therapeutic potential of purified and expanded CD133+ cells human umbilical cord blood (HUCB)-derived in treating myocardial infarction by intramyocardially injecting them into a rat model. Patients who have high cardiovascular risks have fewer endothelial progenitor cells (EPCs) and their EPCs exhibit greater in vitro senescence. HUCB-derived EPCs could be an alternative to rescue impaired stem cell function in the sick and elderly.

The results, which appear in the January 2010 issue of Experimental Biology and Medicine, show that expanded ex vivo exhibited increased expression of mature endothelial cells markers and formed tubule-like structures in vitro. Only the expanded cells expressed VEGF mRNA.

Cells were expanded up to 70-fold during 60 days of culture, and they retained their functional activity. A significant improvement was observed in left ventricular ejection fraction for purified and expanded cells. In summary, CD133+ cells were purified from HUCB, expanded in vitro without losing their biological activity, and both purified and expanded cells showed promising results for use in cellular cardiomyoplasty. However, further pre-clinical testing should be performed to determine whether expanded CD133+ cells have any clinical advantages over purified CD133+ cells.

Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine said "This study suggests that the use of human umbilical cord blood-derived purified and expanded CD133+ cells may show promise for use in cellular cardiomyoplasty. This finding needs subsequent pre-clinical testing but may prove to be very important in future treatments".

Explore further: New urine test could reduce need for blood samples

Related Stories

Recommended for you

Study identifies Ebola virus's Achilles' heel

12 hours ago

An international team including scientists from Albert Einstein College of Medicine of Yeshiva University and the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) has identified the ...

Stem cell therapy for inherited skin blistering

14 hours ago

Promising results from a trial of a new stem-cell based therapy for a rare and debilitating skin condition have been published in the Journal of Investigative Dermatology. The therapy, involving infusions of ste ...

Simple recipe to make sensory hair cells in the ear

14 hours ago

Scientists at the Molecular Medicine Institute in Lisbon, Portugal, and at the University College London Ear Institute, United Kingdom, have developed a simple and efficient protocol to generate inner ear hair cells, the ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.