Knockdown of E2F1 reduces invasive potential of melanoma cells

December 23, 2009

Inhibition of transcription factor E2F1 reduced epidermal growth factor receptor (EGFR) expression and reduced the invasive potential but not proliferation of metastatic melanoma cells, according to a brief communication published online December 23 in the Journal of the National Cancer Institute.

To investigate E2F1's role in progression, Brigitte M. Pützer, M.D., Ph.D., of the department of vectorology and experimental gene therapy at the University Rostock in Germany, and colleagues used E2F1 gene silencing in and in mice to compare cell growth and invasive potential and and formation of metastatic lesions. The authors also examined expression of EGFR, a protein previously found to be associated with cancer progression, and effects of its inhibition.

cells with reduced E2F1 expression had lower invasive potential even though they grew at the same rate as control cells. Tumors in animals with reduced E2F1 expression grew at similar rates, but formed fewer and metastatic lesions than control tumors. EGFR expression was decreased in E2F1-silenced cells, and its inhibition reduced the invasive potential of these cells.

"Because elevated expression of E2F1 and EGFR has been observed in other tumor types, the established mechanistic link may also be important in other human cancers," the authors write. "This association should be explored in future studies."

Study limitations: Because the study was based on specific in vitro and in vivo models, it is still unclear whether these mechanisms are useful targets in human cancer.

Explore further: Alzheimer's enzyme acts as a tumor suppressor

More information: "E2F1 in Melanoma Progression and Metastasis," Journal of the National Cancer Institute, DOI: 10.1093/jnci/djp458

Related Stories

Alzheimer's enzyme acts as a tumor suppressor

June 8, 2007

Researchers at Burnham Institute for Medical Research have provided the first evidence that gamma-secretase, an enzyme key to the progression of Alzheimer’s, acts as a tumor suppressor by altering the pathway of epidermal ...

Recommended for you

How the finch changes its tune

August 3, 2015

Like top musicians, songbirds train from a young age to weed out errors and trim variability from their songs, ultimately becoming consistent and reliable performers. But as with human musicians, even the best are not machines. ...

Machine Translates Thoughts into Speech in Real Time

December 21, 2009

( -- By implanting an electrode into the brain of a person with locked-in syndrome, scientists have demonstrated how to wirelessly transmit neural signals to a speech synthesizer. The "thought-to-speech" process ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.