Scientists create new cloning method

Oct 04, 2006

U.S. scientists say they have achieved a one-step somatic cell nuclear transfer procedure using a differentiated cell as a nuclear donor.

The University of Connecticut researchers say their study demonstrates fully differentiated granulocytes yield cloned mice with greater efficiency than undifferentiated blood stem cells.

Somatic cell nuclear transfer -- SCNT -- is the procedure by which cloned animals are typically produced and involves the injection of a nucleus from a donor cell into an egg whose nucleus has been removed. Previous studies reported cloned mice could only be generated with differentiated cells as nuclear donors using a two-step procedure, in which the early embryos generated by SCNT are first used to generate embryonic stem cells that are subsequently injected into another recipient embryo.

Other studies also reported the cloning efficiency for embryonic stem cells is much higher than for other kinds of cells.

But Xiangzhong Yang and colleagues injected nuclei from blood cells at different stages of differentiation into donor eggs and report the nuclei of granulocytes allow for more efficient production of cloned embryos than blood stem cells.

They say two live pups were born using that procedure.

The experiment appears in the journal Nature Genetics.

Copyright 2006 by United Press International

Explore further: Genetic testing in kids is fraught with complications

Related Stories

Protein plays unexpected role in embryonic stem cells

Jun 18, 2015

What if you found out that pieces of your front door were occasionally flying off the door frame to carry out chores around the house? That's the kind of surprise scientists at the Salk Institute experienced ...

The two faces of cellular forgetfulness

Apr 13, 2015

Ludwig Maximilian University researchers have monitored how epigenetic information is transmitted to daughter cells during cell division and determined when the cell's developmental memory is re-established.

Recommended for you

Genetic testing in kids is fraught with complications

Jul 02, 2015

A woman coping with the burden of familial breast cancer can't help but wonder if her young daughter will suffer the same fate. Has she inherited the same disease-causing mutation? Is it best to be prepared ...

Cause of acute liver failure in young children discovered

Jul 02, 2015

Acute liver failure is a rare yet life-threatening disease for young children. It often occurs extremely rapidly, for example, when a child has a fever. Yet in around 50 percent of cases it is unclear as to why this happens. ...

Genome sequencing illuminates rare Aicardi syndrome

Jul 02, 2015

As my inbox fills with ever more updates on the number of human genomes sequenced and the plummeting time and cost of next next next generation sequencing, I find myself hitting delete more and more often. ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.